Understanding Size-exclusion Chromatography Basics: Choosing the Correct Columns and Detectors

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    Size-exclusion chromatography has become the most popular technique for molecular weight characterization of soluble macromolecules.


    Advancements in both column technology and detector technology have made the technique much more complicated.


    This presentation will present the fundamentals of size-separation, the key performance parameters, and the type of data that can be obtained from this analytical technique.


    Understanding these basic topics will allow for proper columns and chromatographic conditions to be chosen and for the correct molecular data to be obtained from the analysis.



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